Denaturing gradient gel electrophoresis (DGGE) as a tool for identification of marine nematodes
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چکیده
منابع مشابه
Denaturing gradient gel electrophoresis (DGGE) as a tool for identification of marine nematodes
Many phyla of marine invertebrates are difficult to identify using conventional morphological taxonomy. Larvae of a wider set of phyla are also difficult to identify as a result of conservation of morphology between species or because morphological characters are destroyed during sampling and preservation. DNA sequence analysis has the potential for identification of marine organisms to the spe...
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Analysis of microbial communities associated with plants, insects and soil has been a significant challenge for plant protection researchers, because of the lack of techniques with which to access these populations. A new molecular community profiling technique, 16S rRNA gene-based PCR followed by denaturing gradient gel electrophoresis (DGGE), has been used to analyse microbial communities in ...
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We present an online tool (EquiBands, http://www.univie.ac.at/IECB/limno/equibands/EquiBands.html) that quantifies the matching of two bands considered to be the same in different samples, even when samples are applied to different denaturing gradient gel electrophoresis gels. With an environmental example we demonstrate the procedure for the classification of two bands of different samples wit...
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ژورنال
عنوان ژورنال: Marine Ecology Progress Series
سال: 2005
ISSN: 0171-8630,1616-1599
DOI: 10.3354/meps291103